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Product Name
NFE2L1 antibody
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Description
NFE2L1 Rabbit Polyclonal antibody. Positive WB detected in mouse skeletal muscle tissue, Fetal mouse bone, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells. Positive IP detected in mouse skeletal muscle tissue. Observed molecular weight by Western-blot: 65 kDa
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Tested applications
ELISA, WB, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
FLJ00380 antibody; HBZ17 antibody; LCR F1 antibody; LOCus control region factor 1 antibody; NF E2 related factor 1 antibody; NFE2 related factor 1 antibody; NFE2L1 antibody; NRF1 antibody; TCF 11 antibody; TCF11 antibody; Transcription factor 11 antibody; Transcription factor HBZ17 antibody; Transcription factor LCR F1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of NFE2L1 recombinant protein (Accession Number: NM_001330262). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
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Validations
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with Catalog No:113146(NFE2L1 antibody) at dilution of 1:500
IP Result of anti-NFE2L1 (IP:Catalog No:113146, 4ug; Detection:Catalog No:113146 1:500) with mouse skeletal muscle tissue lysate 2200ug.
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Background
NFE2L1, also named as HBZ17, NRF1, TCF11 and LCR-F1, belongs to the bZIP family and CNC subfamily. It activates erythroid-specific, globin gene expression. NFE2L1 is involved in mediating ascorbic acid-induced osterix expression and osteoblast differentiation via binding to the antioxidant response element of the osterix promoter. NFE2L1, produced by osteoblasts, is involved in regulating osterix expression, osteoblast differentiation, and bone formation. (PMID:19887580). This antibody is a rabbit polyclonal antibody raised against a region of human NFE2L1.
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References
- Kim J, Xing W, Wergedal J, Chan JY, Mohan S. Targeted disruption of nuclear factor erythroid-derived 2-like 1 in osteoblasts reduces bone size and bone formation in mice. Physiological genomics. 40(2):100-10. 2010.
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