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Product Name
NFATC3 Polyclonal Antibody
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Description
Polyclonal antibody to NFATC3
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Tested applications
WB, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
NFATC3 antibody; NFAT4 antibody; NFATX antibody; nuclear factor of activated T-cells 3 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-290 of human NFATC3 (NP_775188.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:50 - 1:200 -
Validations
Western blot - NFATC3 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using NFATC3 antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.
Immunofluorescence - NFATC3 Polyclonal Antibody
Immunofluorescence analysis of HeLa cells using NFATC3 antibody . Blue: DAPI for nuclear staining.
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Background
Acts as a regulator of transcriptional activation. Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2. Along with NFATC4, involved in embryonic heart development (By similarity).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"