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Product Name
NF45 antibody
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Description
NF45 Rabbit Polyclonal antibody. Positive WB detected in K-562 cells, HepG2 cells. Positive IF detected in HepG2 cells. Positive IHC detected in human colon tissue, human breast cancer tissue. Observed molecular weight by Western-blot: 43 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human; other species not tested.
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Alternative names
ILF2 antibody; NF45 antibody; PRO3063 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of NF45 recombinant protein (Accession Number: NM_004515). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:50-1:500
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Validations
K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:113139(NF45 Antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human colon slide using Catalog No:113139(NF45 Antibody) at dilution of 1:50
Immunohistochemistry of paraffin-embedded human breast cancer slide using Catalog No:113139(NF45 Antibody) at dilution of 1:100
Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using Catalog No:113139(NF45 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
Interleukin-2 enhancer binding factor 2 (ILF2), also named as nuclear factor 45 (NF45), plays important roles in regulating interleukin-2 expression in mammals. It forms a heterodimeric complex with ILF3, which may regulate transcription of the IL2 gene during T-cell activation. It can also promote the formation of stable DNA-dependent protein kinase holoenzyme complexes on DNA [PMID:11739746,17129403].
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