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Product Name
NF45 antibody
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Description
NF45 Rabbit Polyclonal antibody. Positive IHC detected in human cervical cancer tissue. Positive WB detected in K-562 cells, Jurkat cells, mouse brain tissue, NIH/3T3 cells. Observed molecular weight by Western-blot: 43-45 kDa
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
ILF2 antibody; NF45 antibody; PRO3063 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of NF45 recombinant protein (Accession Number: NM_004515). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
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Validations
K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:113138(NF45 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human cervical cancer using Catalog No:113138(NF45 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human cervical cancer using Catalog No:113138(NF45 antibody) at dilution of 1:100 (under 40x lens)
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Background
Interleukin-2 enhancer binding factor 2 (ILF2), also named as nuclear factor 45 (NF45), plays important roles in regulating interleukin-2 expression in mammals. It forms a heterodimeric complex with ILF3, which may regulate transcription of the IL2 gene during T-cell activation. It can also promote the formation of stable DNA-dependent protein kinase holoenzyme complexes on DNA [PMID:11739746,17129403].
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References
- Yu Y, Pan X, Ding Y. An iTRAQ based quantitative proteomic strategy to explore novel secreted proteins in metastatic hepatocellular carcinoma cell lines. The Analyst. 138(16):4505-11. 2013.
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