-
Product Name
NF-M-Specific antibody
- Documents
-
Description
NF-M-Specific Rabbit Polyclonal antibody. Positive WB detected in mouse brain tissue, human brain tissue, rat brain tissue. Positive IP detected in rat brain tissue. Positive IHC detected in human brain tissue, rat brain tissue. Positive IF detected in Hela cells. Observed molecular weight by Western-blot: 140-160 kDa
-
Tested applications
ELISA, IF, WB, IP, IHC
-
Species reactivity
Human, Mouse, Rat; other species not tested.
-
Alternative names
NEF3 antibody; NEFM antibody; NF M antibody; NFM antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_005382). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:50-1:500
IF: 1:10-1:100
-
Validations
IP Result of anti-NEFM-Specific (IP:Catalog No:113163, 4ug; Detection:Catalog No:113163 1:1000) with rat brain tissue lysate 4000ug.
Immunohistochemistry of paraffin-embedded human brain tissue slide using Catalog No:113163(NEFM-Specific Antibody) at dilution of 1:200 (under 10x lens).
Immunohistochemistry of paraffin-embedded human brain tissue slide using Catalog No:113163(NEFM-Specific Antibody) at dilution of 1:200 (under 40x lens).
Immunofluorescent analysis of Hela cells, using NEFM antibody Catalog No:113163 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
-
Background
NEFM, also named as NEF3 and NFM, belongs to the intermediate filament family. Neurofilaments are the 10nm intermediate filaments found specifically in neurons. They are a major component of the cell's cytoskeleton, and provide support for normal axonal radial growth. Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. The names given to the three major neurofilament subunits are based upon the apparent molecular weight of the mammalian subunits on SDS-PAGE:NF-L, 65-68kd; NF-M,145-160kd and NF-H, 200-220kd.The antibody is specific to NEFM.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"