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Product Name
NF-H antibody
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Description
NF-H Rabbit Polyclonal antibody. Positive WB detected in mouse brain tissue. Positive IHC detected in human brain tissue. Observed molecular weight by Western-blot: 200kd
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of NF-H recombinant protein (Accession Number: NM_021076). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IHC: 1:20-1:200
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:113149(NEFH antibody) at dilution of 1:2000
Immunohistochemistry of paraffin-embedded human brain tissue slide using Catalog No:113149(NEFH Antibody) at dilution of 1:200 (under 10x lens)
Immunohistochemistry of paraffin-embedded human brain tissue slide using Catalog No:113149(NEFH Antibody) at dilution of 1:200 (under 40x lens)
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Background
NEFH, also named as KIAA0845 and NFH, Belongs to the intermediate filament family. It has an important function in mature axons that is not subserved by the two smaller NF proteins. Neurofilaments are the 10nm intermediate filaments found specifically in neurons. They are a major component of the cell's cytoskeleton, and provide support for normal axonal radial growth. Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. The names given to the three major neurofilament subunits are based upon the apparent molecular weight of the mammalian subunits on SDS-PAGE:NF-L, 65-68kd; NF-M,145-160kd and NF-H, 200-220kd. This antibody recognize NEFH only.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"