Product Name

NF-H antibody

Documents

Description

NF-H Rabbit Polyclonal antibody. Positive IHC detected in human oesophagus cancer tissue, human brain tissue. Positive WB detected in mouse brain tissue. Observed molecular weight by Western-blot: 200kd,140-160kd

Tested applications

ELISA, WB, IHC

Species reactivity

Human,Mouse,Rat; other species not tested.

Alternative names

NEFH antibody; NF H antibody; NF200 antibody; NFH antibody

Isotype

Rabbit IgG

Preparation

This antibody was obtained by immunization of NF-H recombinant protein (Accession Number: BC014185). Purification method: Antigen affinity purified.

Clonality

Polyclonal

Formulation

PBS with 0.02% sodium azide and 50% glycerol pH 7.3.

Storage instructions

Store at -20℃. DO NOT ALIQUOT

Applications

Recommended Dilution:

WB: 1:200-1:2000

IHC: 1:20-1:200

Validations

WB result of Catalog No:113148.

mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:113148(NEFH antibody) at dilution of 1:500

Immunohistochemical of paraffin-embedded human oesophagus cancer using Catalog No:113148(NEFH antibody) at dilution of 1:50 (under 10x lens)

Immunohistochemical of paraffin-embedded human oesophagus cancer using Catalog No:113148(NEFH antibody) at dilution of 1:50 (under 40x lens)

Background

NEFH(NF200), also named as KIAA0845 and NFH, Belongs to the intermediate filament family. It has an important function in mature axons that is not subserved by the two smaller NF proteins. Neurofilaments are the 10nm intermediate filaments found specifically in neurons. They are a major component of the cell's cytoskeleton, and provide support for normal axonal radial growth. Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. The names given to the three major neurofilament subunits are based upon the apparent molecular weight of the mammalian subunits on SDS-PAGE:NF-L, 65-68kd; NF-M,145-160kd and NF-H, 200-220kd. This antibody can recognize both NEFH and NEFM.

References

Xu MF, Zhou H, Hu CY, Liang YQ, Hu L, Chen D. The mechanisms of EGFR in the regulation of axon regeneration. Cell biochemistry and function. 32(1):101-5. 2014.
Yin F, Guo L, Meng CY. Transplantation of mesenchymal stem cells exerts anti-apoptotic effects in adult rats after spinal cord ischemia-reperfusion injury. Brain research. 1561:1-10. 2014.
Yin F, Meng C, Lu R. Bone marrow mesenchymal stem cells repair spinal cord ischemia/reperfusion injury by promoting axonal growth and anti-autophagy. Neural regeneration research. 9(18):1665-71. 2014.

NF-H antibody

Product Information

Related Products / Services