Human LCN2/NGAL (His Tag) recombinant protein

Human LCN2/NGAL (His Tag) recombinant protein

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Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth.

Neutrophil gelatinase-associated lipocalin

24P3; SIP24; MSFI; LCN2; AW212229; NGAL; RP23-161B9.11

P80188

LCN2; HNL; NGAL

Human Cells

A DNA sequence encoding the human Lipocalin 2 precursor (NP_005555.2) (Met 1-Gly 198) was expressed with a C-terminal polyhistidine tag.

Human

The secreted recombinant human LCN2 consists of 195 amino acids. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhLCN2 is approximately 22 kDa as predicted.

> 97 % as determined by SDS-PAGE

Measured by its ability to bind Iron(III) dihydroxybenzoic acid [Fe(DHBA)3]. The binding of Fe(DHBA)3 results in the quenching of Trp fluorescence in recombinant human Lipocalin-2. Recombinant human Lipocalin-2 can bind >1.5 μM of Fe(DHBA)3.