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Product Name
Neurotrophin 3 antibody
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Description
Neurotrophin 3 Rabbit Polyclonal antibody. Positive IP detected in SH-SY5Y cells. Positive WB detected in SH-SY5Y cells, human placenta tissue. Positive IHC detected in human brain tissue. Positive IF detected in SH-SY5Y cells. Observed molecular weight by Western-blot: 35 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
HDNF antibody; Nerve growth factor 2 antibody; Neurotrophic factor antibody; neurotrophin 3 antibody; NGF 2 antibody; NGF2 antibody; NT 3 antibody; NT3 antibody; NTF3 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Neurotrophin 3 recombinant protein (Accession Number: NM_002527). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with Catalog No:113133(NTF3 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human brain using Catalog No:113133(NTF3 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human brain using Catalog No:113133(NTF3 antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-NTF3 (IP:Catalog No:113133, 4ug; Detection:Catalog No:113133 1:800) with SH-SY5Y cells lysate 1600ug.
Immunofluorescent analysis of (-20oc Ethanol) fixed SH-SY5Y cells using Catalog No:113133(NTF3 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
NTF3 represents neurotrophin 3
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