Neuropeptide AF (hNPAF), Human /Neuropeptide A-18-F/NPAF

Neuropeptide AF (hNPAF), Human /Neuropeptide A-18-F/NPAF

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

AGEGLNSQFWSLAAPQRF

H-Ala-Gly-Glu-Gly-Leu-Asn-Ser-Gln-Phe-Trp-Ser-Leu-Ala-Ala-Pro-Gln-Arg-Phe-NH2

18

97%

C₉₀H₁₃₂N₂₆O₂₅

1978.2

Synthetic

Powder

This anti-opiate neuropeptide has been implicated in pain modulation as well as in opioid tolerance and may play a critical role in this process. For the neuropeptide Y-like human orphan G protein-coupled receptor HLWAR77 this peptide exhibited an EC50 value of 1.26 nM. Furthermore, it has been shown to modulate a variety of physiological processes such as food intake, insulin release, blood pressure regulation and electrolyte balance. Please see also also our anorexigenic

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• S.J.Perry et al., FEBS Lett., 409, 426 (1997)
• N.A.Elshourbagy et al., J. Biol. Chem., 275, 25965 (2000)
• I.Lefrere et al., J. Biol. Chem., 277, 39169 (2002)

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.