Neuromedin U-25 (human) peptide

Neuromedin U-25 (human) peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

FRVDEEFQSPFASQSRGYFLFRPRN

H-Phe-Arg-Val-Asp-Glu-Glu-Phe-Gln-Ser-Pro-Phe-Ala-Ser-Gln-Ser-Arg-Gly-Tyr-Phe-Leu-Phe-Arg-Pro-Arg-Asn-NH2

25

97.6%

C₁₄₁H₂₀₃N₄₁O₃₈

3080.42

Synthetic

Powder

Neuromedin U is a neuropeptide which is widely distributed in the gut and central nervous system. Initially isolated from porcine spinal cord it was named for its uterine contractile activity. In fasted rats neuromedin U expression in certain brain areas is lower than in fed rats. In animal studies with neuromedin-23 (rat) overnight food intake is significantly reduced indicating that neuromedin U may be a physiological regulator of food intake. IC50 values for the binding

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• M.Honzawa et al., Neuroscience, 23, 1103 (1987)
• J.Ballesta et al., Neuroscience, 25, 797 (1988)
• A.D.Howard et al., Nature, 406, 70 (2000)
• K.Ketterer et al., Cancer Lett., 277, 72 (2008)

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.