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Product Name
Neurogranin antibody
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Description
Neurogranin Rabbit Polyclonal antibody. Positive IHC detected in human brain tissue, human kidney tissue, human lung tissue, human ovary tissue, human placenta tissue, human skin tissue, human spleen tissue, human testis tissue. Positive WB detected in mouse brain tissue. Positive IP detected in mouse brain tissue. Observed molecular weight by Western-blot: 15kd
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
hng antibody; Neurogranin antibody; NRGN antibody; P17 antibody; RC3 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Neurogranin recombinant protein (Accession Number: NM_006176). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:113128(NRGN antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human brain using Catalog No:113128(NRGN antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human brain using Catalog No:113128(NRGN antibody) at dilution of 1:50 (under 40x lens)
IP Result of anti-NRGN (IP:Catalog No:113128, 4ug; Detection:Catalog No:113128 1:1000) with mouse brain tissue lysate 2640ug.
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Background
Neurogranin (formerly designated p17, also known as Ng, RC3 and BICKS) belongs to the neurogranin family. It is a neuron-specific substrate for protein kinase C (PKC). It is a postsynaptic protein that is highly enriched in brain, with restricted expression in the cortex, striatum, hippocampus, thalamus, hypothalamus and olfactory bulb nuclei. Neurogranin binds calmodulin at low levels of calcium, thereby regulating calmodulin-dependent nitric oxide synthase. Neurogranin acts as a "third messenger" substrate of protein kinase C-mediated molecular cascades during synaptic development and remodeling. It binds to calmodulin in the absence of calcium.The observed molecular weight of human NRGN by Western blotting is about 15-17 kDa.
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