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Product Name
NeuN antibody
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Description
NeuN Rabbit Polyclonal antibody. Positive IHC detected in human brain tissue. Positive WB detected in mouse brain tissue, 3T3-L1 cells. Observed molecular weight by Western-blot: 38-50 kDa
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
cDNA FLJ56884 antibody; FLJ56884 antibody; FLJ58356 antibody; FOX3 antibody; hCG_1776007 antibody; HRNBP3 antibody; NeuN antibody; RBFOX3 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of NeuN recombinant protein (Accession Number: NM_001025448). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:113120(NeuN antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human brain using Catalog No:113120(NeuN antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human brain using Catalog No:113120(NeuN antibody) at dilution of 1:50 (under 40x lens)
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Background
NeuN, also named as FOX3 and RBFOX3, is neuronal-specific nuclear protein with MW 38-50kd(~48kd). It is one of a family of 3 mammalian Fox homologues. FOX3(Or NeuN) functions in RNA-binding protein that regulates alternative splicing events.
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