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Product Name
NEU1 Polyclonal Antibody
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Description
Polyclonal antibody to NEU1
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse, Rat
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Alternative names
NEU1 antibody; NANH antibody; NEU antibody; SIAL1 antibody; sialidase-1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 156-415 of human NEU1 (NP_000425.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200 -
Validations
Western blot - NEU1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using NEU1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 3s.
Immunohistochemistry - NEU1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat brain using NEU1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - NEU1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse spinal cord using NEU1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - NEU1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse brain using NEU1 antibody at dilution of 1:100 (40x lens).
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Background
Catalyzes the removal of sialic acid (N-acetylneuraminic acid) moities from glycoproteins and glycolipids. To be active, it is strictly dependent on its presence in the multienzyme complex. Appears to have a preference for alpha 2-3 and alpha 2-6 sialyl linkage.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"