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Product Name
Nestin Polyclonal Antibody
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Description
Polyclonal antibody to Nestin
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Tested applications
WB, IHC, IF, FC
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Species reactivity
Human
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Alternative names
NES antibody; Nbla00170 antibody; nestin antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide of human Nestin
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, pH7.3.
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Storage instructions
Store at 4℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:1000
IHC 1:20 - 1:50
IF 1:20 - 1:50
FC 1:20 - 1:50 -
Validations
Western blot - Nestin Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using Nestin antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 5s.
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Background
Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells (By similarity).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"