Product Name

NELF-A antibody

Documents

Description

NELF-A Rabbit Polyclonal antibody. Positive IP detected in mouse brain tissue. Positive WB detected in HeLa cells, Jurkat cells, mouse brain tissue, mouse liver tissue. Positive IF detected in HeLa cells. Observed molecular weight by Western-blot: 60 kDa

Tested applications

ELISA, IF, WB, IP

Species reactivity

Human, Mouse; other species not tested.

Alternative names

FLJ10442 antibody; FLJ25112 antibody; Negative elongation factor A antibody; NELF A antibody; NELFA antibody; P/OKcl.15 antibody; WHSC2 antibody

Isotype

Rabbit IgG

Preparation

This antibody was obtained by immunization of NELF-A recombinant protein (Accession Number: NM_005663). Purification method: Antigen affinity purified.

Clonality

Polyclonal

Formulation

PBS with 0.1% sodium azide and 50% glycerol pH 7.3.

Storage instructions

Store at -20℃. DO NOT ALIQUOT

Applications

Recommended Dilution:

WB: 1:500-1:5000

IP: 1:200-1:2000

IF: 1:10-1:100

Validations

HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:113109(NELF-A Antibody) at dilution of 1:1000

IP Result of anti-NELF-A (IP:Catalog No:113109, 4ug; Detection:Catalog No:113109 1:500) with mouse brain tissue lysate 3440ug.

Immunofluorescent analysis of HeLa cells using Catalog No:113109(NELF-A Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Background

WHSC2, also known as NELF-A, is a component of the Negative ELongation Factor complex, which inhibits RNA polymerase II progression early during elongation (a process referred to as ‘promoter proximal pausing’), thereby altering expression of its target genes in a positive and negative manner [PMID:19245807]. This complex acts with DRB sensitivity-inducing factor (DSIF), a heterodimer of SPT4 and SPT5, to cause transcriptional pausing of RNA polymerase II[PMID:12612062].

NELF-A antibody

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