NEK8 Polyclonal Antibody

Cat.#: 160198

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Product Information

  • Product Name
    NEK8 Polyclonal Antibody
  • Documents
  • Description
    Polyclonal antibody to NEK8
  • Tested applications
    WB, IF
  • Species reactivity
    Human, Mouse
  • Alternative names
    NEK8 antibody; JCK antibody; NEK12A antibody; NPHP9 antibody; RHPD2 antibody; NIMA related kinase 8 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human NEK8 (NP_835464.1).
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • Storage instructions
    Store at -20℃. Avoid freeze / thaw cycles.
  • Applications
    WB 1:500 - 1:2000
    IF 1:50 - 1:200
  • Validations

    Western blot - NEK8 Polyclonal Antibody

    Western blot - NEK8 Polyclonal Antibody

    Western blot analysis of extracts of various cell lines, using NEK8 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.

    Immunofluorescence - NEK8 Polyclonal Antibody

    Immunofluorescence - NEK8 Polyclonal Antibody

    Immunofluorescence analysis of HeLa cells using NEK8 antibody . Blue: DAPI for nuclear staining.

  • Background
    Required for renal tubular integrity. May regulate local cytoskeletal structure in kidney tubule epithelial cells. May regulate ciliary biogenesis through targeting of proteins to the cilia (By similarity). Plays a role in organogenesis and is involved in the regulation of the Hippo signaling pathway.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"