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Product Name
NEK8 Polyclonal Antibody
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Description
Polyclonal antibody to NEK8
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Tested applications
WB, IF
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Species reactivity
Human, Mouse
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Alternative names
NEK8 antibody; JCK antibody; NEK12A antibody; NPHP9 antibody; RHPD2 antibody; NIMA related kinase 8 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human NEK8 (NP_835464.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:50 - 1:200 -
Validations
Western blot - NEK8 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using NEK8 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.
Immunofluorescence - NEK8 Polyclonal Antibody
Immunofluorescence analysis of HeLa cells using NEK8 antibody . Blue: DAPI for nuclear staining.
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Background
Required for renal tubular integrity. May regulate local cytoskeletal structure in kidney tubule epithelial cells. May regulate ciliary biogenesis through targeting of proteins to the cilia (By similarity). Plays a role in organogenesis and is involved in the regulation of the Hippo signaling pathway.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"