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Product Name
NEDD8 antibody
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Description
NEDD8 Rabbit Polyclonal antibody. Positive IHC detected in human colon cancer tissue. Positive WB detected in mouse brain tissue, human heart tissue. Observed molecular weight by Western-blot: 6-10 kDa
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
cul nedd8 antibody; FLJ43224 antibody; Nedd 8 antibody; NEDD8 antibody; Neddylin antibody; Ubiquitin like protein Nedd8 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of NEDD8 recombinant protein (Accession Number: NM_006156). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:113098(NEDD8 antibody) at dilution of 1:800
Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using Catalog No:113098(NEDD8 Antibody) at dilution of 1:50 (under 40x lens)
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Background
NEDD8 (neural precursor cell-expressed developmentally down-regulated gene 8) is a small ubiquitin-like protein that shares 60% sequence identity and 80% homology with ubiquitin [PMID:9353319]. NEDD8 is conjugated to substrate proteins in a process known as neddylation. NEDD8 forms a thioester bond with APPBP1-Uba3, the E1 enzyme. Activated NEDD8 then transfers to Ubc12, the E2 enzyme . Ultimately, E2 loaded with NEDD8 binds a substrate protein lysine residue and forms an isopeptide bond by E3 ligase [PMID:18802447].
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References
- Lu B, Al-Ramahi I, Valencia A. Identification of NUB1 as a suppressor of mutant Huntington toxicity via enhanced protein clearance. Nature neuroscience. 16(5):562-70. 2013.
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