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Product Name
NEDD4L antibody
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Description
NEDD4L Rabbit Polyclonal antibody. Positive WB detected in K-562 cells, C6 cells, HT-1080 cells, PC-3 cells. Positive IHC detected in human prostate cancer tissue, human prostate hyperplasia tissue, human stomach cancer tissue. Positive IF detected in PC-3 cells. Observed molecular weight by Western-blot: 112 kDa,125 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human, Mouse, Rat; other species not tested.
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Alternative names
FLJ33870 antibody; hNedd4 2 antibody; KIAA0439 antibody; NEDD4 2 antibody; NEDD4.2 antibody; NEDD4L antibody; NEDL3 antibody; RSP5 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of NEDD4L recombinant protein (Accession Number: NM_001243960). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:113097(NEDD4L antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:113097(NEDD4L antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:113097(NEDD4L antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of (-20oc Ethanol) fixed PC-3 cells using Catalog No:113097(NEDD4L Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
NEDD4L(E3 ubiquitin-protein ligase NEDD4-like) is also named as KIAA0439, NEDL3, Nedd4-2. It accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substratesand and is the principal ubiquitin ligase that selectively targets activated SMAD2 and SMAD3 for destruction(PMID:19917253). Nedd4L binds the PY motif of ENaC COOH terminals and catalyzes ubiquitination of the NH2 terminus of the protein for subsequent degradation(PMID:18268134). It has 9 isoforms produced by alternative splicing. This antibody can recognize NEDD4-1 and NEDD4-2.
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References
- Kito Y, Bai J, Goto N. Pathobiological properties of the ubiquitin ligase Nedd4L in melanoma. International journal of experimental pathology. 95(1):24-8. 2014.
- Li Z, Li J, Bi P. Plk1 phosphorylation of PTEN causes a tumor-promoting metabolic state. Molecular and cellular biology. 34(19):3642-61. 2014.
- Gao C, Pang L, Ren C, Ma T. Decreased expression of Nedd4L correlates with poor prognosis in gastric cancer patient. Medical oncology (Northwood, London, England). 29(3):1733-8. 2012.
- Takeuchi T, Adachi Y, Nagayama T, Furihata M. Nedd4L modulates the transcription of metalloproteinase-1 and -13 genes to increase the invasive activity of gallbladder cancer. International journal of experimental pathology. 92(2):79-86. 2011.
- Skouloudaki K, Walz G. YAP1 recruits c-Abl to protect angiomotin-like 1 from Nedd4-mediated degradation. PloS one. 7(4):e35735. 2012.
- Jung S, Li C, Jeong D. Oncogenic function of p34SEI-1 via NEDD4‑1‑mediated PTEN ubiquitination/degradation and activation of the PI3K/AKT pathway. International journal of oncology. 43(5):1587-95. 2013.
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