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Product Name
NECAB3 Polyclonal Antibody
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Description
Polyclonal antibody to NECAB3
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Tested applications
WB
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Species reactivity
Human, Mouse, Rat
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Alternative names
NECAB3 antibody; APBA2BP antibody; EFCBP3 antibody; NIP1 antibody; STIP3 antibody; SYTIP2 antibody; XB51 antibody; dJ63M2.4 antibody; dJ63M2.5 antibody; N-terminal EF-hand calcium-binding protein 3 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-362 of human NECAB3 (NP_112508.3).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:200 - 1:2000
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Validations
Western blot - NECAB3 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using NECAB3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.
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Background
Inhibits the interaction of APBA2 with amyloid-beta precursor protein (APP), and hence allows formation of amyloid-beta. May enhance the activity of HIF1A and thus promote glycolysis under normoxic conditions; the function requires its ABM domain and may implicate the stabilization of the interaction between HIF1AN and APBA3.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"