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Product Name
NDUFS7 antibody
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Description
NDUFS7 Rabbit Polyclonal antibody. Positive WB detected in human brain tissue, K-562 cells. Positive IHC detected in human liver tissue. Positive IF detected in Hela cells. Observed molecular weight by Western-blot: 20kd
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
CI 20KD antibody; Complex I 20kD antibody; FLJ45860 antibody; FLJ46880 antibody; MY017 antibody; NDUFS7 antibody; PSST antibody; PSST subunit antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of NDUFS7 recombinant protein (Accession Number: NM_024407). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:113085(NDUFS7 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human liver using Catalog No:113085(NDUFS7 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human liver using Catalog No:113085(NDUFS7 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of Hela cells, using NDUFS7 antibody Catalog No:113085 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
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Background
NDUFS7(NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial) protein is one of the most conserved subunits of mitochondrial respiratory chain complex I and plays a central role in the interaction with the electron acceptor ubiquinone and in the proton-translocating mechanism(PMID:15269216).Defects in NDUFS7 are a cause of Leigh syndrome (LS) and mitochondrial complex I deficiency (MT-C1D).
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References
- Frykman S, Teranishi Y, Hur JY. Identification of two novel synaptic γ-secretase associated proteins that affect amyloid β-peptide levels without altering Notch processing. Neurochemistry international. 61(1):108-18. 2012.
- Inoue M, Hur JY, Kihara T. Human brain proteins showing neuron-specific interactions with γ-secretase. The FEBS journal. 282(14):2587-99. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"