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Product Name
NDUFS5 antibody
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Description
NDUFS5 Rabbit Polyclonal antibody. Positive IHC detected in human heart tissue. Positive WB detected in mouse liver tissue, L02 cells, mouse heart tissue. Observed molecular weight by Western-blot: 15 kDa
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
CI 15 kDa antibody; Complex I 15 kDa antibody; NDUFS5 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of NDUFS5 recombinant protein (Accession Number: NM_001184979). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
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Validations
WB result of Catalog No:113083(anti-NDUFS5) in mouse Tissue by Dr. Pawel Grzmil.
mouse liver tissue were subjected to SDS PAGE followed by western blot with Catalog No:113083(NDUFS5 antibody) at dilution of 1:800
Immunohistochemical of paraffin-embedded human heart using Catalog No:113083(NDUFS5 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human heart using Catalog No:113083(NDUFS5 antibody) at dilution of 1:50 (under 40x lens)
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Background
NDUFS5 gene is a member of the NADH dehydrogenase (ubiquinone) iron-sulfur protein family. The encoded protein is a subunit of the NADH:ubiquinone oxidoreductase (complex I), the first enzyme complex in the electron transport chain located in the inner mitochondrial membrane. The NDUFS5 mRNA is expressed ubiquitously in human tissues, with a relative higher expression in human heart, skeletal muscle, liver, kidney and fetal heart (PMID: 10070614). The NDUFS5 (15 kDa) subunit located at mitochondrion and contains two C-X9-C motifs that are predicted to form a helix-coil-helix structure, permitting the formation of intramolecular disulfide bonds (PMID: 21310150). Proteinteach’s NDUFS5 antibody 15224-1-AP is capable of detecting the 15 kDa protein in Western blotting.
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