NDUFS5 antibody

Cat.#: 113083

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Product Information

  • Product Name
    NDUFS5 antibody
  • Documents
  • Description
    NDUFS5 Rabbit Polyclonal antibody. Positive IHC detected in human heart tissue. Positive WB detected in mouse liver tissue, L02 cells, mouse heart tissue. Observed molecular weight by Western-blot: 15 kDa
  • Tested applications
    ELISA, WB, IHC
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    CI 15 kDa antibody; Complex I 15 kDa antibody; NDUFS5 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of NDUFS5 recombinant protein (Accession Number: NM_001184979). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:2000

    IHC: 1:20-1:200

  • Validations

    WB result of Catalog No:113083(anti-NDUFS5) in mouse Tissue by Dr. Pawel Grzmil.

    WB result of Catalog No:113083(anti-NDUFS5) in mouse Tissue by Dr. Pawel Grzmil.

    mouse liver tissue were subjected to SDS PAGE followed by western blot with Catalog No:113083(NDUFS5 antibody) at dilution of 1:800

    mouse liver tissue were subjected to SDS PAGE followed by western blot with Catalog No:113083(NDUFS5 antibody) at dilution of 1:800

    Immunohistochemical of paraffin-embedded human heart using Catalog No:113083(NDUFS5 antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemical of paraffin-embedded human heart using Catalog No:113083(NDUFS5 antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemical of paraffin-embedded human heart using Catalog No:113083(NDUFS5 antibody) at dilution of 1:50 (under 40x lens)

    Immunohistochemical of paraffin-embedded human heart using Catalog No:113083(NDUFS5 antibody) at dilution of 1:50 (under 40x lens)

  • Background
    NDUFS5 gene is a member of the NADH dehydrogenase (ubiquinone) iron-sulfur protein family. The encoded protein is a subunit of the NADH:ubiquinone oxidoreductase (complex I), the first enzyme complex in the electron transport chain located in the inner mitochondrial membrane. The NDUFS5 mRNA is expressed ubiquitously in human tissues, with a relative higher expression in human heart, skeletal muscle, liver, kidney and fetal heart (PMID: 10070614). The NDUFS5 (15 kDa) subunit located at mitochondrion and contains two C-X9-C motifs that are predicted to form a helix-coil-helix structure, permitting the formation of intramolecular disulfide bonds (PMID: 21310150). Proteinteach’s NDUFS5 antibody 15224-1-AP is capable of detecting the 15 kDa protein in Western blotting.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"