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Product Name
NDUFC2 Polyclonal Antibody
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Description
Polyclonal antibody to NDUFC2
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse, Rat
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Alternative names
NDUFC2 antibody; B14.5b antibody; CI-B14.5b antibody; HLC-1 antibody; NADHDH2 antibody; NADH:ubiquinone oxidoreductase subunit C2 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human NDUFC2 (NP_004540.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:100 - 1:200 -
Validations
Western blot - NDUFC2 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using NDUFC2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 5s.
Western blot - NDUFC2 Polyclonal Antibody
Western blot analysis of extracts from normal (control) and NDUFC2 knockout HeLa cells, using NDUFC2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 3min.
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Background
Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"