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Product Name
NDUFB8 antibody
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Description
NDUFB8 Rabbit Polyclonal antibody. Positive IF detected in HeLa cells. Positive IHC detected in human liver tissue, human heart tissue. Positive WB detected in mouse liver tissue, 3T3-L1 cells, HepG2 cells, human heart tissue, mouse brain tissue, mouse cerebellum tissue, mouse heart tissue, mouse skeletal muscle tissue. Positive IP detected in mouse liver tissue. Observed molecular weight by Western-blot: 19-22 kDa
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Tested applications
ELISA, WB, IHC, IP, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
ASHI antibody; CI ASHI antibody; Complex I ASHI antibody; NDUFB8 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of NDUFB8 recombinant protein (Accession Number: NM_005004). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
mouse liver tissue were subjected to SDS PAGE followed by western blot with Catalog No:113077(NDUFB8 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human liver using Catalog No:113077(NDUFB8 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human liver using Catalog No:113077(NDUFB8 antibody) at dilution of 1:50 (under 40x lens)
IP Result of anti-NDUFB8 (IP:Catalog No:113077, 4ug; Detection:Catalog No:113077 1:1000) with mouse liver tissue lysate 4000ug.
Immunofluorescent analysis of (-20oc Ethanol) fixed HeLa cells using Catalog No:113077(NDUFB8 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
NDUFB8 represents NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 8, 19kDa
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