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Product Name
NDUFAF2 antibody
- Documents
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Description
NDUFAF2 Rabbit Polyclonal antibody. Positive IHC detected in human liver tissue, human skeletal muscle tissue. Positive IF detected in HepG2 cells. Positive WB detected in HepG2 cells, HeLa cells, mouse skeletal muscle tissue, SH-SY5Y cells. Positive IP detected in SH-SY5Y cells. Observed molecular weight by Western-blot: 25 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
B17.2 like antibody; B17.2L antibody; FLJ22398 antibody; mimitin antibody; Mimitin antibody; mitOChondrial antibody; MMTN antibody; NDUFA12 like protein antibody; NDUFA12L antibody; NDUFAF2 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of NDUFAF2 recombinant protein (Accession Number: NM_174889). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:113067(NDUFAF2 antibody) at dilution of 1:800
Immunohistochemical of paraffin-embedded human liver using Catalog No:113067(NDUFAF2 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human liver using Catalog No:113067(NDUFAF2 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using Catalog No:113067 and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
IP Result of anti-NDUFAF2 (IP:Catalog No:113067, 4ug; Detection:Catalog No:113067 1:500) with SH-SY5Y cells lysate 1600ug.
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Background
Mimitin, also named as MMTN, B17.2L, NDUFAF2 and NDUFA12L, is a small mitochondrial protein upregulated by proinflammatory cytokines at the transcriptional and protein levels. It is reported as a possible chaperone for assembly of mitochondrial complex I. The MW of Mimitin is 17-25 kDa.
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References
- Ostergaard E, Rodenburg RJ, van den Brand M. Respiratory chain complex I deficiency due to NDUFA12 mutations as a new cause of Leigh syndrome. Journal of medical genetics. 48(11):737-40. 2011.
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