-
Product Name
NDUFA7 Polyclonal Antibody
- Documents
-
Description
Polyclonal antibody to NDUFA7
-
Tested applications
WB, IHC
-
Species reactivity
Human, Mouse, Rat
-
Alternative names
NDUFA7 antibody; B14.5a antibody; CI-B14.5a antibody; NADH:ubiquinone oxidoreductase subunit A7 antibody
-
Isotype
Rabbit IgG
-
Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-113 of human NDUFA7 (NP_004992.2).
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
-
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
-
Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:100 -
Validations
Western blot - NDUFA7 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using NDUFA7 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.
Western blot - NDUFA7 Polyclonal Antibody
Western blot analysis of extracts from normal (control) and NDUFA7 knockout 293T cells, using NDUFA7 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 5s.
Immunohistochemistry - NDUFA7 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human liver injury using NDUFA7 antibody at dilution of 1:100 (40x lens).
-
Background
Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"