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Product Name
NDUFA6 Polyclonal Antibody
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Description
Polyclonal antibody to NDUFA6
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse, Rat
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Alternative names
NDUFA6 antibody; B14 antibody; CI-B14 antibody; LYRM6 antibody; NADHB14 antibody; NADH:ubiquinone oxidoreductase subunit A6 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 35-154 of human NDUFA6 (NP_002481.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:100 -
Validations
Western blot - NDUFA6 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using NDUFA6 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunohistochemistry - NDUFA6 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse lung using NDUFA6 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - NDUFA6 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human stomach using NDUFA6 antibody at dilution of 1:100 (40x lens).
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Background
Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed to be not involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"