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Product Name
NDUFA5 antibody
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Description
NDUFA5 Rabbit Polyclonal antibody. Positive WB detected in human brain tissue, HeLa cells, human heart tissue, rat liver tissue. Positive IHC detected in human brain tissue. Observed molecular weight by Western-blot: 13kd
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
B13 antibody; CI 13KD B antibody; Complex I 13kD B antibody; Complex I subunit B13 antibody; DKFZp781K1356 antibody; FLJ12147 antibody; NDUFA5 antibody; NUFM antibody; UQOR13 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of NDUFA5 recombinant protein (Accession Number: NM_005000). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
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Validations
human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:113061(NDUFA5 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human brain using Catalog No:113061(NDUFA5 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human brain using Catalog No:113061(NDUFA5 antibody) at dilution of 1:50 (under 40x lens)
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Background
NDUFA5, also named as CI-13kD-B and NDUA5, belongs to the complex I NDUFA5 subunit family. It is accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which functions in the transfer of electrons from NADH to the respiratory chain. NDUFA5,an enrichment of mitochondrial protein, can be used as a mitochondrial marker protein(PMID:21360670).
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References
- Egan B, Dowling P, O'Connor PL. 2-D DIGE analysis of the mitochondrial proteome from human skeletal muscle reveals time course-dependent remodelling in response to 14 consecutive days of endurance exercise training. Proteomics. 11(8):1413-28. 2011.
- Shiau JY, Yin SY, Chang SL. Mechanistic Study of the Phytocompound, 2- β -D-Glucopyranosyloxy-1-hydroxytrideca-5,7,9,11-tetrayne in Human T-Cell Acute Lymphocytic Leukemia Cells by Using Combined Differential Proteomics and Bioinformatics Approaches. Evidence-based complementary and alternative medicine : eCAM. 2015:475610. 2015.
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