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NDFIP1 antibody

Cat.#: 113055

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Product Information

  • Product Name
    NDFIP1 antibody
  • Documents
  • Description
    NDFIP1 Rabbit Polyclonal antibody. Positive WB detected in HEK-293 cells. Positive IP detected in HEK-293 cells. Positive IF detected in HEK-293 cells. Observed molecular weight by Western-blot: 30 kDa
  • Tested applications
    ELISA, IP, WB, IF
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    N4WBP5 antibody; NDFIP1 antibody; PSEC0223 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of NDFIP1 recombinant protein (Accession Number: NM_030571). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:1000

    IP: 1:200-1:1000

    IF: 1:20-1:200

  • Validations

    HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:113055(NDFIP1 Antibody) at dilution of 1:300

    HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:113055(NDFIP1 Antibody) at dilution of 1:300

    IP Result of anti-NDFIP1 (IP:Catalog No:113055, 3ug; Detection:Catalog No:113055 1:300) with HEK-293 cells lysate 3200ug.

    IP Result of anti-NDFIP1 (IP:Catalog No:113055, 3ug; Detection:Catalog No:113055 1:300) with HEK-293 cells lysate 3200ug.

    Immunofluorescent analysis of HEK-293 cells using Catalog No:113055(NDFIP1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

    Immunofluorescent analysis of HEK-293 cells using Catalog No:113055(NDFIP1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

  • Background
    Ndfip1 is an adaptor protein for the Nedd4 family of ubiquitin ligases and has been found to be upregulated in neurons after brain injury, including head trauma, stroke and metal toxicity. Recent studies confirmed the role of Ndfip1 as a regulator of iron metabolism and pointed out that Ndfip1 was involved in iron homeostasis by regulating the degradation of iron importer divalent metal transporter 1. Ndfip1 is a transmembrane protein that is localized to the Golgi and post-Golgi vesicles such as endosomes. It is an adaptor protein that recruits E3 ligases to ubiquitinate target proteins. It is ubiquitously expressed and contains 2 PY motifs, which interact with several Nedd4 family E3s to degradate proteins.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"