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Product Name
NDE1 antibody
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Description
NDE1 Rabbit Polyclonal antibody. Positive WB detected in A2780 cells, HEK-293 cells, HeLa cells, mouse liver tissue, rat liver tissue. Positive IP detected in HEK-293 cells. Positive IHC detected in human breast cancer tissue, human colon cancer tissue. Positive IF detected in MCF-7 cells. Observed molecular weight by Western-blot: 40kd
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
FLJ20101 antibody; HOM TES 87 antibody; NDE1 antibody; NUDE antibody; NUDE1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of NDE1 recombinant protein (Accession Number: NM_001143979). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
A2780 cells were subjected to SDS PAGE followed by western blot with Catalog No:113053(NDE1 antibody) at dilution of 1:1500
IP Result of anti-NDE1 (IP:Catalog No:113053, 3ug; Detection:Catalog No:113053 1:1500) with HEK-293 cells lysate 4500ug.
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:113053(NDE1 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:113053(NDE1 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of MCF-7 cells, using NDE1 antibody Catalog No:113053 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
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Background
NDE1 (NUDE /NUDE1) is a LIS1-interacting protein. A signaling pathway containing NDE1, LIS1, and cytoplasmic dynein is conserved for eukaryotic nuclear migration and has profound impact on neuronal migration during development of the central nervous system. LIS1 and dynein have been shown to be involved in mitosis and NDE1 participates in a subset of dynein function in M phase. NDE1 is specifically phosphorylated in M phase in its serine/threonine phosphorylation motifs, probably by Cdc2 and also Erk1 and Erk2. A fraction of NDEl binds to centrosomes strongly in interphase and is localized to mitotic spindles in early M phase. Phosphorylation of NDE1 also regulates the cell-cycle-dependent distribution, possibly by increasing its dissociation rate at the microtubule-organizing center. This antibody is a rabbit polyclonal antibody raised against a peptide mapping within human NDE1.
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References
- Kim S, Zaghloul NA, Bubenshchikova E. Nde1-mediated inhibition of ciliogenesis affects cell cycle re-entry. Nature cell biology. 13(4):351-60. 2011.
- Vergnolle MA, Taylor SS. Cenp-F links kinetochores to Ndel1/Nde1/Lis1/dynein microtubule motor complexes. Current biology : CB. 17(13):1173-9. 2007.
- Houlihan SL, Feng Y. The scaffold protein Nde1 safeguards the brain genome during S phase of early neural progenitor differentiation. eLife. 3:e03297. 2014.
- Chan YW, Fava LL, Uldschmid A. Mitotic control of kinetochore-associated dynein and spindle orientation by human Spindly. The Journal of cell biology. 185(5):859-74. 2009.
- Lam C, Vergnolle MA, Thorpe L, Woodman PG, Allan VJ. Functional interplay between LIS1, NDE1 and NDEL1 in dynein-dependent organelle positioning. Journal of cell science. 123(Pt 2):202-12. 2010.
- Bakircioglu M, Carvalho OP, Khurshid M. The essential role of centrosomal NDE1 in human cerebral cortex neurogenesis. American journal of human genetics. 88(5):523-35. 2011.
- Maskey D, Marlin MC, Kim S. Cell cycle-dependent ubiquitylation and destruction of NDE1 by CDK5-FBW7 regulates ciliary length. The EMBO journal. 34(19):2424-40. 2015.
- Inaba H, Goto H, Kasahara K. Ndel1 suppresses ciliogenesis in proliferating cells by regulating the trichoplein-Aurora A pathway. The Journal of cell biology. 212(4):409-23. 2016.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"