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Product Name
NDC80 antibody
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Description
NDC80 Rabbit Polyclonal antibody. Positive IHC detected in human brain tissue. Positive WB detected in A2780 cells, human spleen tissue. Positive IP detected in A2780 cells. Observed molecular weight by Western-blot: 76 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
HEC antibody; HEC1 antibody; HsHec1 antibody; hsNDC80 antibody; KinetOChore protein Hec1 antibody; KNTC2 antibody; NDC80 antibody; TID3 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of NDC80 recombinant protein (Accession Number: NM_006101). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
A2780 cells were subjected to SDS PAGE followed by western blot with Catalog No:113052(NDC80 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human brain using Catalog No:113052(NDC80 antibody) at dilution of 1:100 (under 10x lens)
IP Result of anti-NDC80 (IP:Catalog No:113052, 4ug; Detection:Catalog No:113052 1:500) with A2780 cells lysate 960ug.
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Background
NDC80 represents NDC80 homolog, kinetochore complex component (S. cerevisiae)
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References
- Liu Y, Hu H, Zhang C. Co-expression of mitosis-regulating genes contributes to malignant progression and prognosis in oligodendrogliomas. Oncotarget. 6(35):38257-69. 2015.
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