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Product Name
NCOR1 Polyclonal Antibody
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Description
Polyclonal antibody to NCOR1
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Tested applications
WB, IF
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Species reactivity
Human
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Alternative names
NCOR1 antibody; N-CoR antibody; N-CoR1 antibody; PPP1R109 antibody; TRAC1 antibody; hN-CoR antibody; nuclear receptor corepressor 1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human NCOR1 (NP_006302.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:50 - 1:100 -
Validations
Western blot - NCOR1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using NCOR1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunofluorescence - NCOR1 Polyclonal Antibody
Immunofluorescence analysis of U2OS cells using NCOR1 antibody . Blue: DAPI for nuclear staining.
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Background
Mediates transcriptional repression by certain nuclear receptors. Part of a complex which promotes histone deacetylation and the formation of repressive chromatin structures which may impede the access of basal transcription factors. Participates in the transcriptional repressor activity produced by BCL6. Recruited by ZBTB7A to the androgen response elements/ARE on target genes, negatively regulates androgen receptor signaling and androgen-induced cell proliferation.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"