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Product Name
NCOA5 antibody
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Description
NCOA5 Rabbit Polyclonal antibody. Positive WB detected in HEK-293 cells, HeLa cells. Positive IP detected in HEK-293 cells. Positive FC detected in HEK-293 cells. Positive IF detected in Hela cells. Observed molecular weight by Western-blot: 66 kDa
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Tested applications
ELISA, WB, IF, IP, FC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
bA465L10.6 antibody; CIA antibody; KIAA1637 antibody; NCoA 5 antibody; NCOA5 antibody; nuclear receptor coactivator 5 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of NCOA5 recombinant protein (Accession Number: BC056872). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IF: 1:10-1:100
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Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:113043(NCOA5 antibody) at dilution of 1:600
Immunofluorescent analysis of Hela cells, using NCOA5 antibody Catalog No:113043 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-NCOA5 (IP:Catalog No:113043, 4ug; Detection:Catalog No:113043 1:800) with HEK-293 cells lysate 3000ug.
1X10^6 HEK-293 cells were stained with 0.2ug NCOA5 antibody (Catalog No:113043, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.
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Background
Nuclear receptor coactivator 5 (NCOA5), also known as coactivator independent of AF-2 (CIA), possesses both repressor and activator functions (PMID: 11113208). It can interact with Estrogen Receptor (ER) alpha and ER beta in a ligand-dependent manner. NCOA5 has been shown to regulate the ER alpha-mediated transcription as well as the expression of the c-Myc gene (PMID: 11113208; 15073177). Recently identified as a conserved component of pluripotent stem cells, mammalian NCOA5 may contribute to the maintenance of the pluripotent stem cell population (PMID: 24268775).
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References
- Böser A, Drexler HC, Reuter H. SILAC proteomics of planarians identifies Ncoa5 as a conserved component of pluripotent stem cells. Cell reports. 5(4):1142-55. 2013.
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