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Product Name
NCOA2 Polyclonal Antibody
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Description
Polyclonal antibody to NCOA2
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse, Rat
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Alternative names
NCOA2 antibody; GRIP1 antibody; KAT13C antibody; NCoA-2 antibody; SRC2 antibody; TIF2 antibody; bHLHe75 antibody; nuclear receptor coactivator 2 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 550-820 of human NCOA2 (NP_006531.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200 -
Validations
Western blot - NCOA2 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using NCOA2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 90s.
Western blot - NCOA2 Polyclonal Antibody
Western blot analysis of extracts from normal (control) and NCOA2 knockout 293T cells, using NCOA2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 3min.CRISPR KO cell lines generated by
Immunohistochemistry - NCOA2 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat brain using NCOA2 antibody at dilution of 1:100 (40x lens).
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Background
Transcriptional coactivator for steroid receptors and nuclear receptors. Coactivator of the steroid binding domain (AF-2) but not of the modulating N-terminal domain (AF-1). Required with NCOA1 to control energy balance between white and brown adipose tissues. Critical regulator of glucose metabolism regulation, acts as RORA coactivator to specifically modulate G6PC expression. Involved in the positive regulation of the transcriptional activity of the glucocorticoid receptor NR3C1 by sumoylation enhancer RWDD3. Positively regulates the circadian clock by acting as a transcriptional coactivator for the CLOCK-ARNTL/BMAL1 heterodimer (By similarity).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"