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Product Name
NCKAP1 antibody
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Description
NCKAP1 Rabbit Polyclonal antibody. Positive IHC detected in human gliomas tissue, human heart tissue. Positive WB detected in mouse brain tissue, HepG2 cells, human brain tissue. Observed molecular weight by Western-blot: 115-125 kDa
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
FLJ11291 antibody; HEM2 antibody; KIAA0587 antibody; NAP 1 antibody; NAP1 antibody; NAP125 antibody; NCK assOCiated protein 1 antibody; NCKAP1 antibody; p125Nap1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of NCKAP1 recombinant protein (Accession Number: NM_013436). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:113038(NAP1;NCKAP1 antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human heart tissue slide using Catalog No:113038(NAP1;NCKAP1 Antibody) at dilution of 1:50 (under 40x lens)
Immunohistochemistry of paraffin-embedded human gliomas tissue slide using Catalog No:113038(NAP1;NCKAP1 Antibody) at dilution of 1:50 (under 40x lens)
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Background
NCK-associated protein 1 (NCKAP1), also known as NAP1 or HEM2, is encoded by an apoptosis-related gene human NAP1, the expression of which is strongly down-regulated in sporadic Alzheimer's disease (AD). Human NAP1 is predominantly expressed in neuronal cells. Antisense oligo DNA of human NAP1 transcripts was found to induce apoptosis of neuronal cells. Loss of NAP1 function disrupts neuronal differentiation. Likewise, NAP1 plays an essential role in facilitating neuronal cytoskeletal changes underlying the postmigratory differentiation of cortical neurons, a critical step in functional wiring of the cortex. Human NAP1 is also proved to be an orthologue of rat Nap1 which binds to the adaptor molecule NCK in signal transduction.
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References
- Wu S, Ma L, Wu Y, Zeng R, Zhu X. Nudel is crucial for the WAVE complex assembly in vivo by selectively promoting subcomplex stability and formation through direct interactions. Cell research. 22(8):1270-84. 2012.
- Turanov AA, Shchedrina VA, Everley RA. Selenoprotein S is involved in maintenance and transport of multiprotein complexes. The Biochemical journal. 462(3):555-65. 2014.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"