NCK1 Polyclonal Antibody

Cat.#: 160023

Special Price 290.3 USD

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Product Information

  • Product Name
    NCK1 Polyclonal Antibody
  • Documents
  • Description
    Polyclonal antibody to NCK1
  • Tested applications
    WB, IHC
  • Species reactivity
    Human
  • Alternative names
    NCK1 antibody; NCK antibody; NCKalpha antibody; nck-1 antibody; NCK adaptor protein 1 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: A synthetic peptide of human NCK1
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide, pH7.3.
  • Storage instructions
    Store at 4℃. Avoid freeze / thaw cycles.
  • Applications
    WB 1:500 - 1:2000
    IHC 1:50 - 1:100
  • Validations

    Western blot - NCK1 Polyclonal Antibody

    Western blot - NCK1 Polyclonal Antibody

    Western blot analysis of extracts of various cell lines, using NCK1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.

  • Background
    Adapter protein which associates with tyrosine-phosphorylated growth factor receptors, such as KDR and PDGFRB, or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in the DNA damage response, not in the detection of the damage by ATM/ATR, but for efficient activation of downstream effectors, such as that of CHEK2. Plays a role in ELK1-dependent transcriptional activation in response to activated Ras signaling. Modulates the activation of EIF2AK2/PKR by dsRNA. May play a role in cell adhesion and migration through interaction with ephrin receptors.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"