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Product Name
NCF4 antibody
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Description
NCF4 Rabbit Polyclonal antibody. Positive IHC detected in human heart tissue. Positive WB detected in L02 cells. Observed molecular weight by Western-blot: 41 kDa
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
NCF antibody; NCF 4 antibody; NCF4 antibody; Neutrophil cytosol factor 4 antibody; p40 phox antibody; P40PHOX antibody; SH3PXD4 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of NCF4 recombinant protein (Accession Number: NM_013416). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IHC: 1:20-1:200
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Validations
L02 cells were subjected to SDS PAGE followed by western blot with Catalog No:113035(NCF4 Antibody) at dilution of 1:300
Immunohistochemical of paraffin-embedded human heart using Catalog No:113035(NCF4 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human heart using Catalog No:113035(NCF4 antibody) at dilution of 1:50 (under 40x lens)
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Background
NCF4, also named as p40phox, was the last NADPH oxidase subunit to be discovered, and although there is evidence of its both positive and negative effects on oxidase function. Catalog#14648-1-AP is a rabbit polyclonal antibody raised against the full-length of human NCF4.The MW of this protein is 40 kDa, and this antibody specially recognises the 40 kDa protein.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"