NCF1 Polyclonal Antibody

Cat.#: 160754

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Product Information

  • Product Name
    NCF1 Polyclonal Antibody
  • Documents
  • Description
    Polyclonal antibody to NCF1
  • Tested applications
    WB, IF, IP
  • Species reactivity
    Human, Mouse, Rat
  • Alternative names
    NCF1 antibody; NCF1A antibody; NOXO2 antibody; SH3PXD1A antibody; p47phox antibody; neutrophil cytosol factor 1 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-390 of human NCF1 (NP_000256.4).
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • Storage instructions
    Store at -20℃. Avoid freeze / thaw cycles.
  • Applications
    WB 1:500 - 1:2000
    IF 1:50 - 1:100
    IP 1:50 - 1:100
  • Validations

    Western blot - NCF1 Polyclonal Antibody

    Western blot - NCF1 Polyclonal Antibody

    Western blot analysis of extracts of various cell lines, using NCF1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.

    Immunoprecipitation - NCF1 Polyclonal Antibody

    Immunoprecipitation - NCF1 Polyclonal Antibody

    Immunoprecipitation analysis of 200ug extracts of Raji cells, using 3 ug NCF1 antibody . Western blot was performed from the immunoprecipitate using NCF1 antibody at a dilition of 1:1000.

  • Background
    NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"