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Product Name
NCF1 Polyclonal Antibody
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Description
Polyclonal antibody to NCF1
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Tested applications
WB, IF, IP
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Species reactivity
Human, Mouse, Rat
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Alternative names
NCF1 antibody; NCF1A antibody; NOXO2 antibody; SH3PXD1A antibody; p47phox antibody; neutrophil cytosol factor 1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-390 of human NCF1 (NP_000256.4).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:50 - 1:100
IP 1:50 - 1:100 -
Validations
Western blot - NCF1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using NCF1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.
Immunoprecipitation - NCF1 Polyclonal Antibody
Immunoprecipitation analysis of 200ug extracts of Raji cells, using 3 ug NCF1 antibody . Western blot was performed from the immunoprecipitate using NCF1 antibody at a dilition of 1:1000.
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Background
NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"