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NCBP1 antibody

Cat.#: 113034

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Product Information

  • Product Name
    NCBP1 antibody
  • Documents
  • Description
    NCBP1 Rabbit Polyclonal antibody. Positive IHC detected in human lung cancer tissue, human kidney tissue. Positive WB detected in HEK-293 cells, HeLa cells, human brain tissue. Positive IP detected in HeLa cells. Observed molecular weight by Western-blot: 80kd
  • Tested applications
    ELISA, WB, IHC, IP
  • Species reactivity
    Human, Mouse; other species not tested.
  • Alternative names
    CBP80 antibody; NCBP antibody; NCBP 80 kDa subunit antibody; NCBP1 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of NCBP1 recombinant protein (Accession Number: NM_002486). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IP: 1:200-1:2000

    IHC: 1:20-1:200

  • Validations

    HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:113034(CBP80 antibody) at dilution of 1:1000

    HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:113034(CBP80 antibody) at dilution of 1:1000

    Immunohistochemical of paraffin-embedded human lung cancer using Catalog No:113034(CBP80 antibody) at dilution of 1:100 (under 10x lens)

    Immunohistochemical of paraffin-embedded human lung cancer using Catalog No:113034(CBP80 antibody) at dilution of 1:100 (under 10x lens)

    IP Result of anti-CBP80 (IP:Catalog No:113034, 4ug; Detection:Catalog No:113034 1:500) with HeLa cells lysate 2480ug.

    IP Result of anti-CBP80 (IP:Catalog No:113034, 4ug; Detection:Catalog No:113034 1:500) with HeLa cells lysate 2480ug.

  • Background
    Nuclear cap binding protein subunit 1, 80kDa (NCBP1, synonyms: NCBP, CBP80) is a subunit of a cap-binding protein complex (CBC) that consists of a CBP20 subunit, which binds the cap, and a NCBP1 subunit, which ensures high-affinity cap binding. The CBC complex may play a role in pre-mRNA recognition and splicing.
  • References
    • Fustin JM, Doi M, Yamaguchi Y. RNA-methylation-dependent RNA processing controls the speed of the circadian clock. Cell. 155(4):793-806. 2013.
    • Sato H, Hosoda N, Maquat LE. Efficiency of the pioneer round of translation affects the cellular site of nonsense-mediated mRNA decay. Molecular cell. 29(2):255-62. 2008.
    • Yamashita A, Izumi N, Kashima I. SMG-8 and SMG-9, two novel subunits of the SMG-1 complex, regulate remodeling of the mRNA surveillance complex during nonsense-mediated mRNA decay. Genes & development. 23(9):1091-105. 2009.
    • Matsuda D, Sato H, Maquat LE. Chapter 9. Studying nonsense-mediated mRNA decay in mammalian cells. Methods in enzymology. 449:177-201. 2008.
    • Izumi N, Yamashita A, Iwamatsu A. AAA+ proteins RUVBL1 and RUVBL2 coordinate PIKK activity and function in nonsense-mediated mRNA decay. Science signaling. 3(116):ra27. 2010.
    • Izumi N, Yamashita A, Hirano H, Ohno S. Heat shock protein 90 regulates phosphatidylinositol 3-kinase-related protein kinase family proteins together with the RUVBL1/2 and Tel2-containing co-factor complex. Cancer science. 103(1):50-7. 2012.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"