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Product Name
NCAPG antibody
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Description
NCAPG Rabbit Polyclonal antibody. Positive IP detected in HEK-293 cells. Positive WB detected in HEK-293 cells, HeLa cells. Positive IF detected in HeLa cells. Positive IHC detected in human testis tissue, human cervical cancer tissue. Observed molecular weight by Western-blot: 131 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human; other species not tested.
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Alternative names
CAPG antibody; CHCG antibody; Condensin complex subunit 3 antibody; Condensin subunit CAP G antibody; FLJ12450 antibody; HCAP G antibody; Melanoma antigen NY MEL 3 antibody; NCAPG antibody; NY MEL 3 antibody; NYMEL3 antibody; XCAP G homolog antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of NCAPG recombinant protein (Accession Number: NM_022346). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:113031(NCAPG Antibody) at dilution of 1:1500
IP Result of anti-NCAPG (IP:Catalog No:113031, 4ug; Detection:Catalog No:113031 1:1000) with HEK-293 cells lysate 3200ug.
Immunohistochemistry of paraffin-embedded human cervical cancer tissue slide using Catalog No:113031(NCAPG Antibody) at dilution of 1:50 (under 40x lens)
Immunohistochemistry of paraffin-embedded human testis tissue slide using Catalog No:113031(NCAPG Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HeLa cells using Catalog No:113031(NCAPG Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
NCAPG represents non-SMC condensin I complex, subunit G
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