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Product Name
NAT2 Polyclonal Antibody
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Description
Polyclonal antibody to NAT2
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
NAT2 antibody; AAC2 antibody; NAT-2 antibody; PNAT antibody; N-acetyltransferase 2 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 171-290 of human NAT2 (NP_000006.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200 -
Validations
Western blot - NAT2 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using NAT2 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 30s.
Immunohistochemistry - NAT2 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat lung using NAT2 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - NAT2 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat heart using NAT2 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - NAT2 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human breast using NAT2 antibody at dilution of 1:100 (40x lens).
Immunofluorescence - NAT2 Polyclonal Antibody
Immunofluorescence analysis of 293T cells using NAT2 antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Background
Participates in the detoxification of a plethora of hydrazine and arylamine drugs. Catalyzes the N- or O-acetylation of various arylamine and heterocyclic amine substrates and is able to bioactivate several known carcinogens.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"