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Product Name
NAT10 antibody
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Description
NAT10 Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, human brain tissue, mouse spleen tissue, mouse testis tissue. Positive IP detected in mouse testis tissue. Positive IF detected in Hela cells. Observed molecular weight by Western-blot: 116 kDa
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Tested applications
ELISA, IF, IP, WB
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
ALP antibody; DKFZp434C116 antibody; FLJ10774 antibody; FLJ12179 antibody; FLJ23850 antibody; KIAA1709 antibody; N acetyltransferase 10 antibody; NAT10 antibody; NET43 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of NAT10 recombinant protein (Accession Number: NM_024662). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:200-1:2000
IF: 1:20-1:200
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Validations
Immunofluorescent analysis of Hela cells, using NAT10 antibody Catalog No:112961 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:112961(NAT10 Antibody) at dilution of 1:3000
IP Result of anti-NAT10 (IP:Catalog No:112961, 2ug; Detection:Catalog No:112961 1:500) with mouse testis tissue lysate 4000ug.
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Background
NAT10 (N-acetyltransferase 10) is a nucleolar protein that is involved in regulation of telomerase activity, DNA damage response, and cytokinesis. It also plays a role in maintaining nuclear shape. Inhibition of NAT10 has been reported to rescue the misshapen nuclei in laminopathic cells via microtubule reorganization. The specificity of this antibody has been tested by siRNA. (24786082)
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References
- Larrieu D, Britton S, Demir M, Rodriguez R, Jackson SP. Chemical inhibition of NAT10 corrects defects of laminopathic cells. Science (New York, N.Y.). 344(6183):527-32. 2014.
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