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Product Name
NASP Polyclonal Antibody
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Description
Polyclonal antibody to NASP
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
NASP antibody; FLB7527 antibody; HMDRA1 antibody; PRO1999 antibody; nuclear autoantigenic sperm protein antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 180-449 of human NASP (NP_689511.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200 -
Validations
Western blot - NASP Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using NASP antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunohistochemistry - NASP Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human breast cancer using NASP Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - NASP Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse liver using NASP Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - NASP Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse spleen using NASP Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - NASP Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat spleen using NASP Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - NASP Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat stomach using NASP Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - NASP Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human tonsil using NASP Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - NASP Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human appendicitis using NASP Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - NASP Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human brain astrocytoma using NASP Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - NASP Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human breast using NASP Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - NASP Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human breast cancer using NASP Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - NASP Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human stomach using NASP Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - NASP Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human gastric cancer using NASP Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - NASP Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human leiomyoma of uterus using NASP Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - NASP Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human adenomyosis using NASP Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - NASP Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse lung using NASP Antibody at dilution of 1:100 (40x lens).
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Background
Required for DNA replication, normal cell cycle progression and cell proliferation. Forms a cytoplasmic complex with HSP90 and H1 linker histones and stimulates HSP90 ATPase activity. NASP and H1 histone are subsequently released from the complex and translocate to the nucleus where the histone is released for binding to DNA.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"