Product Name

NASP antibody

Documents

Description

NASP Rabbit Polyclonal antibody. Positive IHC detected in human testis tissue, human lymphoma tissue. Positive IF detected in MCF-7 cells. Positive IP detected in mouse testis tissue. Positive WB detected in mouse testis tissue. Observed molecular weight by Western-blot: 138kd

Tested applications

ELISA, WB, IHC, IF, IP

Species reactivity

Human,Mouse,Rat; other species not tested.

Alternative names

DKFZp547F162 antibody; FLB7527 antibody; FLJ31599 antibody; FLJ35510 antibody; NASP antibody; PRO1999 antibody

Isotype

Rabbit IgG

Preparation

This antibody was obtained by immunization of NASP recombinant protein (Accession Number: NM_002482). Purification method: Antigen affinity purified.

Clonality

Polyclonal

Formulation

PBS with 0.1% sodium azide and 50% glycerol pH 7.3.

Storage instructions

Store at -20℃. DO NOT ALIQUOT

Applications

Recommended Dilution:

WB: 1:500-1:5000

IP: 1:500-1:5000

IHC: 1:20-1:200

IF: 1:10-1:100

Validations

mouse testis tissue were subjected to SDS PAGE followed by western blot with Catalog No:112958(NASP antibody) at dilution of 1:1000

Immunohistochemical of paraffin-embedded human testis using Catalog No:112958(NASP antibody) at dilution of 1:100 (under 10x lens)

Immunohistochemical of paraffin-embedded human testis using Catalog No:112958(NASP antibody) at dilution of 1:100 (under 40x lens)

Immunofluorescent analysis of MCF-7 cells, using NASP antibody Catalog No:112958 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

IP Result of anti-NASP (IP:Catalog No:112958, 5ug; Detection:Catalog No:112958 1:1000) with mouse testis tissue lysate 4000ug.

Background

NASP (nuclear autoantigenic sperm protein) is associated with DNA replication, cell proliferation and cell cycle progression through functioning as a Histone H1 binding protein that mediates histone transport to the nucleus. NASP had two isoforms, tNASP (testis type) and sNASP (somatic type). The tNASP was mainly expressed in testis, a variety of malignant tumors, stem cells and embryonic tissues, while sNASP existed in all somatic mitosis cells. This antibody can recognize both isoforms.

References

Campos EI, Fillingham J, Li G. The program for processing newly synthesized histones H3.1 and H4. Nature structural & molecular biology. 17(11):1343-51. 2010.
Kato K, Okuwaki M, Nagata K. Role of Template Activating Factor-I as a chaperone in linker histone dynamics. Journal of cell science. 124(Pt 19):3254-65. 2011.
Ma W, Xie S, Ni M. MicroRNA-29a inhibited epididymal epithelial cell proliferation by targeting nuclear autoantigenic sperm protein (NASP). The Journal of biological chemistry. 287(13):10189-99. 2012.
Nishibu T, Hayashida Y, Tani S. Identification of MIWI-associated Poly(A) RNAs by immunoprecipitation with an anti-MIWI monoclonal antibody. Bioscience trends. 6(5):248-61. 2012.
Yuan Q, Han J, Cong W. Docetaxel-loaded solid lipid nanoparticles suppress breast cancer cells growth with reduced myelosuppression toxicity. International journal of nanomedicine. 9:4829-46. 2014.
Drané P, Ouararhni K, Depaux A, Shuaib M, Hamiche A. The death-associated protein DAXX is a novel histone chaperone involved in the replication-independent deposition of H3.3. Genes & development. 24(12):1253-65. 2010.

NASP antibody

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