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Product Name
NAPRT1 antibody
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Description
NAPRT1 Rabbit Polyclonal antibody. Positive WB detected in HEK-293 cells, HepG2 cells, human colon tissue, human small intestine tissue, L02 cells, mouse kidney tissue, mouse liver tissue. Positive IP detected in HEK-293 cells. Observed molecular weight by Western-blot: 51 kDa, 56 kDa
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Tested applications
ELISA, WB, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
FHA HIT interacting protein antibody; FHIP antibody; NAPRT1 antibody; NAPRTase antibody; PP3856 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of NAPRT1 recombinant protein (Accession Number: NM_001363146). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
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Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:112949(NAPRT1 antibody) at dilution of 1:1000
IP Result of anti-NAPRT1 (IP:Catalog No:112949, 4ug; Detection:Catalog No:112949 1:1000) with HEK-293 cells lysate 2800ug.
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Background
Nicotinic acid (NA) is a coenzyme in cellular redox reactions, and is an essential component of metabolic pathways in all living cells. NAPRT1 (Nicotinate phosphoribosyltransferase) is essential for increasing cellular NAD levels and, thus, to prevent oxidative stress of cells. NAPRT1 converts Nicotinic acid (NA; niacin) to NA mononucleotide (NaMN), which is then converted to NA adenine dinucleotide (NaAD), and finally to nicotinamide adenine dinucleotide (NAD).
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References
- Watson M, Roulston A, Bélec L. The small molecule GMX1778 is a potent inhibitor of NAD+ biosynthesis: strategy for enhanced therapy in nicotinic acid phosphoribosyltransferase 1-deficient tumors. Molecular and cellular biology. 29(21):5872-88. 2009.
- Olesen UH, Hastrup N, Sehested M. Expression patterns of nicotinamide phosphoribosyltransferase and nicotinic acid phosphoribosyltransferase in human malignant lymphomas. APMIS : acta pathologica, microbiologica, et immunologica Scandinavica. 119(4-5):296-303. 2011.
- Cerna D, Li H, Flaherty S, Takebe N, Coleman CN, Yoo SS. Inhibition of nicotinamide phosphoribosyltransferase (NAMPT) activity by small molecule GMX1778 regulates reactive oxygen species (ROS)-mediated cytotoxicity in a p53- and nicotinic acid phosphoribosyltransferase1 (NAPRT1)-dependent manner. The Journal of biological chemistry. 287(26):22408-17. 2012.
- Chini CC, Guerrico AM, Nin V. Targeting of NAD metabolism in pancreatic cancer cells: potential novel therapy for pancreatic tumors. Clinical cancer research : an official journal of the American Association for Cancer Research. 20(1):120-30. 2014.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"