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Product Name
NANOG antibody
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Description
NANOG Rabbit Polyclonal antibody. Positive IF detected in human embronic stem cells. Positive IP detected in NCCIT cell. Positive WB detected in rat brain tissue, human brain tissue, mouse brain tissue. Observed molecular weight by Western-blot: 43-48 kDa, 66-70 kDa
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Tested applications
ELISA, IF, WB, IP
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Species reactivity
Human, Mouse, Rat; other species not tested.
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Alternative names
hNanog antibody; Homeobox protein NANOG antibody; NANOG antibody; Nanog homeobox antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of NANOG recombinant protein (Accession Number: NM_024865). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:500-1:5000
IF: 1:20-1:200
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Validations
Confocal immunofluorescent analysis of human embronic stem cells with Catalog No:113020 at dilution of 1:200. The PE shows staining with Catalog No:113020/PE. The DAPI shows nuclear staining by DAPI.
rat brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:113020(NANOG Antibody) at dilution of 1:2000
IP Result of anti-NANOG (IP:Catalog No:113020, 4ug; Detection:Catalog No:113020 1:1500) with NCCIT cell lysate 2000ug.
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Background
Nanog is a member of the homeobox family of DNA binding transcription factors and has been shown to maintain embryonic stem (ES) cell self-renewal independently of leukemia inhibitory factor (LIF)/Stat3. Nanog mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Functionally, Nanog works together with other key pluripotent factors (oct4, Sox2, and Lin28) to reprogram human fibroblasts and generate induced pluripotent stem (iPS) cells. These key factors form a regulatory network to support or limit each other's expression level, which maintains the properties of ES cells. Affinity purified rabbit anti-Nanog can be used to demonstrate pluripotency of ES and IPS cells. There are two kinds of variants could recognized by NANOG, one is normal form (~39kd), the other is PTD form (~48kd) (21136380 )
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References
- Qian P, Banerjee A, Wu ZS. Loss of SNAIL regulated miR-128-2 on chromosome 3p22.3 targets multiple stem cell factors to promote transformation of mammary epithelial cells. Cancer research. 72(22):6036-50. 2012.
- Wu Y, Shu J, He C. ROCK inhibitor Y27632 promotes proliferation and diminishes apoptosis of marmoset induced pluripotent stem cells by suppressing expression and activity of caspase 3. Theriogenology. 85(2):302-14. 2016.
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