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Product Name
NAGA antibody
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Description
NAGA Rabbit Polyclonal antibody. Positive IHC detected in human placenta tissue. Positive IF detected in HeLa cells. Positive WB detected in HeLa cells. Observed molecular weight by Western-blot: 48 kDa,60-70 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Alpha galactosidase B antibody; D22S674 antibody; GALB antibody; NAGA antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of NAGA recombinant protein (Accession Number: NM_001362850). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:113015(NAGA antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human placenta using Catalog No:113015(NAGA antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human placenta using Catalog No:113015(NAGA antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of HeLa cells using Catalog No:113015(NAGA Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
NAGA belongs to the glycosyl hydrolase 27 family. It removes terminal alpha-N-acetylgalactosamine residues from glycolipids and glycopeptides. It is required for the breakdown of glycolipids. Biosynthetic studies performed with cultured fibroblasts indicated that the human enzyme was synthesized as a 65kDa glycosylated precursor which was processed to a mature 48-kDa lysosomal form; both the precursor and mature forms had high mannose type oligosaccharide chains, but only the precursor’s mannose residues were phosphorylated. 90-117kd is a homodimer of NAGA.
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