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Product Name
NADK antibody
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Description
NADK Rabbit Polyclonal antibody. Positive WB detected in RAW 264.7 cells, HeLa cells, NIH/3T3 cells. Positive IF detected in Hela cells. Positive IHC detected in human kidney tissue, human brain tissue, human lung tissue, human ovary tissue, human testis tissue. Observed molecular weight by Western-blot: 49 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
dJ283E3.1 antibody; FLJ13052 antibody; FLJ37724 antibody; NAD kinase antibody; NADK antibody; Poly(P)/ATP NAD kinase antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of NADK recombinant protein (Accession Number: XM_005244778). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
RAW264.7 cells were subjected to SDS PAGE followed by western blot with Catalog No:113014(NADK antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human kidney using Catalog No:113014(NADK antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human kidney using Catalog No:113014(NADK antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of Hela cells, using NADK antibody Catalog No:113014 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
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Background
NAD kinase (NADK) is the sole NADP+-biosynthetic enzyme that catalyzes phosphorylation of NAD+ to yield NADP+ using ATP as a phosphoryl donor, and thus, plays a vital role in the cell and represents a potentially powerful antimicrobial drug target(PMID:21526340). It belongs to the NAD kinase family. The deduced 446-amino acid protein has a calculated molecular mass of 49.2 kD. The catalytically active human NADK is a homotetramer(PMID:11594753).
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