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Product Name
NA14 antibody
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Description
NA14 Rabbit Polyclonal antibody. Positive IHC detected in human colon cancer. Positive WB detected in mouse testis tissue, mouse thymus tissue, human testis tissue. Observed molecular weight by Western-blot: 14kd
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Tested applications
ELISA, WB, IHC
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Species reactivity
human, mouse, rat; other species not tested.
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Alternative names
SSNA1 antibody; NA 14 antibody; NA14 antibody; N14 antibody; Nuclear autoantigen of 14 kDa antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of NA14 recombinant protein (Accession Number: NM_003731). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
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Validations
mouse testis tissue were subjected to SDS PAGE followed by western blot with Catalog No:113010(NA14 antibody) at dilution of 1:400
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:113010(NA14 antibody) at dilution of 1:50 (under 10x lens)
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Background
SSNA1, also named as NA14 and DIP13, may be an important adaptor to target spastin activity at the centrosome. SSNA1 is a 14 kDa protetin which locates the basal bodies and flagella of human sperm cells and the centrosome of cells.
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References
- Nitta T, Tam R, Kim JW, Fan H. The cellular protein La functions in enhancement of virus release through lipid rafts facilitated by murine leukemia virus glycosylated Gag. mBio. 2(1):e00341-10. 2011.
- Lai CK, Gupta N, Wen X. Functional characterization of putative cilia genes by high-content analysis. Molecular biology of the cell. 22(7):1104-19. 2011.
- Nishibu T, Hayashida Y, Tani S. Identification of MIWI-associated Poly(A) RNAs by immunoprecipitation with an anti-MIWI monoclonal antibody. Bioscience trends. 6(5):248-61. 2012.
- Goyal U, Renvoisé B, Chang J, Blackstone C. Spastin-interacting protein NA14/SSNA1 functions in cytokinesis and axon development. PloS one. 9(11):e112428. 2014.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"