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Product Name
MYST2 antibody
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Description
MYST2 Rabbit Polyclonal antibody. Positive IF detected in NIH/3T3 cells. Positive IHC detected in human ovary tumor tissue, human breast cancer tissue, mouse kidney tissue. Positive IP detected in HeLa cells. Positive WB detected in NIH/3T3 cells, HeLa cells, human testis tissue, Jurkat cells, MCF7 cells, PC-3 cells. Observed molecular weight by Western-blot: 83 kDa
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Tested applications
ELISA, WB, IHC, IP, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
HBO1 antibody; HBOA antibody; KAT7 antibody; MYST 2 antibody; MYST2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of MYST2 recombinant protein (Accession Number: NM_007067). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:50-1:500
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Validations
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with Catalog No:113003(MYST2 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human ovary tumor using Catalog No:113003(MYST2 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human ovary tumor using Catalog No:113003(MYST2 antibody) at dilution of 1:50 (under 40x lens)
IP Result of anti-MYST2 (IP:Catalog No:113003, 4ug; Detection:Catalog No:113003 1:500) with HeLa cells lysate 2480ug.
Immunofluorescent analysis of (10% Formaldehyde) fixed NIH/3T3 cells using Catalog No:113003(MYST2 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
MYST2, also named as HBO1, HBOa MOZ, YBF2/SAS3, SAS2 and TIP60 protein 2, belongs to the MYST (SAS/MOZ) family. It specifically represses AR mediated transcription. MYST2 is a candidate oncogene. It enhances the anchorage-independent growth of breast cancer cells.(PMID:19372580) MYST2 is a histone acetyltransferase (HAT) which could exert oncogenic function in breast cancer. It is an important downstream molecule of ERa, and ERK1/2 signaling pathway may involved in the expression of HBO1 increased by E2.
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References
- Wang WZ, Liu HO, Wu YH. Estrogen receptor α (ERα) mediates 17β-estradiol (E2)-activated expression of HBO1. Journal of experimental & clinical cancer research : CR. 29:140. 2010.
- Yu H, Diao H, Wang C. Acetylproteomic analysis reveals functional implications of lysine acetylation in human spermatozoa (sperm). Molecular & cellular proteomics : MCP. 14(4):1009-23. 2015.
- Wang D, Fang C, Zong NC. Regulation of acetylation restores proteolytic function of diseased myocardium in mouse and human. Molecular & cellular proteomics : MCP. 12(12):3793-802. 2013.
- Duong MT, Akli S, Macalou S. Hbo1 is a cyclin E/CDK2 substrate that enriches breast cancer stem-like cells. Cancer research. 73(17):5556-68. 2013.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"